Dog Breeding Pre-Mate Test Pads - Case Studies
On-going Study
If you have a case study - normal or abnormal that is well documented,
feel free to submit it to Lapdog along with the Pre-Mate Pad photos.

Case Study 1 - Normal (Bitch A)

Case Study 1 and Case Study 2 are related studies in that both bitches live together and cycle together in their seasons and have done so for nearly two years.  What makes these two cases interesting during the cycle recorded here is that Bitch B in Case Study 2 came into season first.  She appeared to be normal in her season and her LH rise was in the timeframe expected.  However on her 10th day and at the beginning of her LH spike, the second bitch, Bitch A in Case Study 1 came on heat.  The progesterone rise was slowed down and Bitch B started heavy bleeding, which she had not done during the first 10 days of her season.  Meanwhile, Bitch A in Case Study 1 proceeded along normally and the rise in LH was as expected and the pad stayed pink in the PM on day 11.  On day 12, she registered 4.9 on her blood progesterone test.  Ovulation is considered to be when the bitch registers 5 on progesterone, so we can assume this was Bitch A's ovulation day.  Blood progesterone was measured from Bitch B on the same day as Bitch A -- this was Day 21 for Bitch B and her progesterone was almost exactly the same as Bitch A, registering 5.4.  It appears that Bitch A's hormones were more dominate and influenced Bitch B to such a degree that Bitch B had, what is termed, a split season in that she had gone along normally until Bitch A came on heat and then "cooled off" and as Bitch A released her LH, Bitch A and B's progesterone rose together at the same rates.  The Dog Breeding Pre-Mate Test Pads have a note that they are not reliable with split seasons.  We have included the pad results in Case 2 showing the results on a split season - in this case, caused by a second bitch with a stronger hormonal influence coming on heat 10 days after the first bitch.  Bitch A was mated and produced a litter of 10 puppies on her 63rd day of pregnancy.

Date	Cycle Day	Notes	Pad AM	Pad PM
Monday, 28 June	1	Heavy bleeding
Tuesday, 29 June	2	Heavy bleeding
Wednesday, 30 June	3	Heavy bleeding
Thursday, 1 July	4	Heavy bleeding
Friday, 2 July	5	Heavy bleeding
Saturday, 3 July	6	Heavy bleeding
Sunday, 4 July	7	Heavy bleeding
Monday, 5 July	8	Heavy bleeding  - Pre-Mate Pad small line of purple at top of pad
Tuesday, 6 July	9	Heavy bleeding.  Pre-Mate Pad purple on edges in AM.  More over all purple in PM.		spike
Wednesday, 7 July	10	Heavy bleeding - red.  Pre-Mate Pad overall purple colour AM, not as much PM.	spike
Thursday, 8 July	11	Bleeding slowing down some, still red.  Hint of purple on Pre-Mate Pad in AM.  Tissue above vulva still firm.
Friday, 9 July	12	Bleeding red, slowed down.  Touch of purple AM.  Stayed pink PM.  Tissue above vulva soft. Ovulation.  Blood Progesterone 4.9 at 10 AM / Thyroid T4 - 19 (normal range 10 to 36)
Saturday, 10 July	13
Sunday, 11 July	14	1st Mating 6.30 PM - slip mating then 20 minute tie
Monday, 12 July	15	2nd Mating 4.30 PM - 20 minute tie
Tuesday, 13 July	16	3rd Mating 4.30 PM - 20 minute tie
Wednesday, 14 July	17
Thursday, 15 July	18
Friday, 16 July	19
Saturday, 17 July	20
Sunday, 18 July	21
Monday, 19 July	22

Case Study 2 - Abnormal Cycle (Bitch B)

Split season.  See notes under Case Study 1

Date	Cycle Day	Notes	Pad AM	Pad PM
Saturday, 19 June	1	Bleeding moderately - red colour
Sunday, 20 June	2	No noticeable blood
Monday, 21 June	3	No noticeable blood
Tuesday, 22 June	4	No noticeable blood
Wednesday, 23 June	5	No noticeable blood
Thursday, 24 June	6	No noticeable blood
Friday, 25 June	7	No noticeable blood
Saturday, 26 June	8	No noticeable blood
Sunday, 27 June	9	No noticeable blood
Monday, 28 June	10	No noticeable blood  - Pre-Mate Test Pad turned purple at both 9 AM and 9 PM.  2nd bitch (Case Study 1) in same kennel started season today.	spike
Tuesday, 29 June	11	Pre-Mate Test Pad turned purple 9.30 AM and 9 PM	spike
Wednesday, 30 June	12	Pre-Mate Test Pad turned purple around edges 8.45 Am and 11.30 PM.  Noticeable dark red blood.
Thursday, 1 July	13	Pre-Mate Test Pad - small amount of purple both AM / PM.  Blood dark red and noticeable.  Blood progesterone 1.9 / 10 AM.  PM tissue above vulva spongy soft.
Friday, 2 July	14	Blood turning brighter red.  Small amount of purple around the edges of pad.  Blood progesterone 2.3 / 10 AM
Saturday, 3 July	15	Bleeding moderately heavy - red blood
Sunday, 4 July	16	Bleeding moderately heavy - red blood
Monday, 5 July	17	Bleeding moderately - red blood.  Blood progesterone 2.5 / 10 AM / Thyroid T4 = 15 (normal 10 to 35)  Previous season T4 was 18.
Tuesday, 6 July	18	Still bleeding red, but appears to have slowed
Wednesday, 7 July	19	Still bleeding red, but slowing down
Thursday, 8 July	20	Still bleeding red, but slowing down.  Tissue above vulva soft.  PM - first square 60 seconds, second square 2 minutes
Friday, 9 July	21	Bleeding slowed down.  Tissue above vulva soft.  Ovulation.  Blood Progesterone - 5.4 at 10 AM (tested same time as Case Study 1 bitch who was 4.9  on her 12th day).  Pad stayed pink AM, but touch of purple PM.  As noted on instructions - pads are not reliable with split seasons.
Saturday, 10 July	22
Sunday, 11 July	23	Not mated
Monday, 12 July	24
Tuesday, 13 July	25
Wednesday, 14 July	26
Thursday, 15 July	27
Friday, 16, July	28

Case Study 3

Date	Cycle Day	Notes	Pad AM	Pad PM
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Case Study 4

Date	Cycle Day	Notes	Pad AM	Pad PM
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